![]() Liu H, Ding Y, Zhou Y et al (2017) CRISPR-P 2.0: an improved CRISPR-Cas9 tool for genome editing in plants. Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Li X, Xie Y, Zhu Q, Liu Y-G (2017) Targeted genome editing in genes and cis-regulatory regions improves qualitative and quantitative traits in crops. Ito Y, Nishizawa-Yokoi A, Endo M et al (2015) CRISPR/Cas9-mediated mutagenesis of the RIN locus that regulates tomato fruit ripening. Isaacson T, Ronen G, Zamir D, Hirschberg J (2002) Cloning of tangerine from tomato reveals a carotenoid isomerase essential for the production of β-carotene and xanthophylls in plants. ![]() Hsiau T, Conant D, Rossi N et al (2019) Inference of CRISPR edits from Sanger trace data. Hirschberg J (2001) Carotenoid biosynthesis in flowering plants. Higo K, Ugawa Y, Iwamoto M, Korenaga T (1999) Plant cis-acting regulatory DNA elements (PLACE) database: 1999. Gidoni D, Fuss E, Burbidge A et al (2003) Multi-functional T-DNA/Ds tomato lines designed for gene cloning and molecular and physical dissection of the tomato genome. įraser PD, Bramley PM (2004) The biosynthesis and nutritional uses of carotenoids. (96)80019-7ĭoudna JA, Charpentier E (2014) The new frontier of genome engineering with CRISPR-Cas9. ĭemmig-Adams B, Adams WW (1996) The role of xanthophyll cycle carotenoids in the protection of photosynthesis. ĭellaPenna D, Pogson BJ (2006) Vitamin synthesis in plants: tocopherols and carotenoids. ĭahan-Meir T, Filler-Hayut S, Melamed-Bessudo C et al (2018) Efficient in planta gene targeting in tomato using geminiviral replicons and the CRISPR/Cas9 system. Ĭucu T, Huvaere K, Van Den Bergh M-A et al (2012) A Simple and fast HPLC method to determine lycopene in foods. Stereochemistry of poly-cis lycopene and its congeners in ‘ tangerine’ tomato fruits. Ĭlough JM, Pattenden G (1979) Naturally occurring poly-cis carotenoids. ![]() Ĭhen Y, Li F, Wurtzel ET (2010) Isolation and characterization of the Z-ISO gene encoding a missing component of carotenoid biosynthesis in plants. Ĭhaudhary P, Sharma A, Singh B, Nagpal AK (2018) Bioactivities of phytochemicals present in tomato. Ĭarbone F, Pizzichini D, Giuliano G et al (2005) Comparative profiling of tomato fruits and leaves evidences a complex modulation of global transcript profiles. īurton-Freeman B, Reimers K (2011) Tomato consumption and health: emerging benefits. oryzae reveals differential activities for SWEET14-inducing TAL effectors. īlanvillain-Baufumé S, Reschke M, Solé M et al (2017) Targeted promoter editing for rice resistance to Xanthomonas oryzae pv. Environ Exp Bot 32:85–100īelhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V (2013) Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. īarnes J, Balaguer L, Manrique E et al (1992) A reappraisal of the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants. īao A, Burritt DJ, Chen H et al (2019) The CRISPR/Cas9 system and its applications in crop genome editing. Molecular and biochemical studies demonstrate that CRISPR-mediated point mutations in the exon sequence lead to complete knockout of protein function whereas deletion in 5′ UTR region lowers the expression of the gene leading to changes in plant phenotype.Īnzalone AV, Randolph PB, Davis JR et al (2019) Search-and-replace genome editing without double-strand breaks or donor DNA. Mutations were generated in the 5′ UTR and exon 1 of the carotenoid isomerase gene using CRISPR/Cas9 expression via Agrobacterium-mediated transformation of tomato variety Periyakulam 1 (PKM1). ![]() In the current study, we demonstrate this on tomato carotenoid isomerase, a key gene in the carotenoid biosynthesis pathway. Mutations in the coding and regulatory regions can have varying impacts on the function of the gene. Such targeted changes are finding wide application in human health as well as in precision breeding of crop plants for improved traits. AbstractĬRISPR/Cas9 based genome editing is an effective and useful tool adopted from the bacterial immune response system for altering specific, pre-determined DNA sequences in eukaryotes. A deletion created by CRISPR/Cas9 system in the 5’ UTR of the carotenoid isomerase gene in tomato leads to downregulation of the gene resulting in the low conversion of prolycopene to lycopene. ![]()
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